PSC HOMEPSC LaboratoriesEnglish / JapanesePlant metabolomics intends to identify and determine the all plant metabolites in a context of the functional genomics study. We perform high-throughput metabolite analyses and develop technologies for the comprehensive analysis of metabolites with high-performance mass spectrometers.
News
Press Release (14/Nov/2008)
Construction of MS/MS spectral tag (MS2T) library-based peak annotation procedure for informative non-targeted metabolic profiling analysis using liquid chromatography-mass spectrometry (LC-MS).
Fumio Matsuda, Keiko Yonekura-Sakakibara, Rie Niida, Takashi Kuromori, Kazuo Shinozaki and Kazuki Saito (2008) MS/MS spectral tag-based annotation of non-targeted profile of plant secondary metabolites. Plant J. 10.1111/j.1365-313X.2008.03705.x
[Release text]
Content of Research
- Development of analytical techniques for mass spectrometry
- Database construction and bioinformatics for metabolomic data analysis
- Metabolomic analysis for plant biology through collaboration with other groups
Result of Research
- he metabolomics pipeline was constructed for the comprehensive analysis of various plant metabolites including amino acids, organic acids, nucleotides, complex lipid and secondary metabolites. The pipeline consists of GC (gas chromatography)-, CE (capillary electrophoresis)-, and LC (liquid chromatography)-MS branches with a data processing techniques based on the multivariate analysis
- Large number of chromatogram and mass spectrum of plant metabolites were collected for an information basis of the plant metabolome analysis. The data were open to the public via MassBank, a mass spectrum database (JST/BIRD).
- Behaviors of the plant primary metabolism were compared among the wild-type and metabolic mutants of Arabidopsis by the metabolite-metabolites correlation network analysis. The data mining with a combination of transcriptome data revealed the candidate genes responsible for the regulation of primary metabolism in plants.
- The function of flavanoid and anthocyanin biosynthesis related genes of Arabidopsis were identified by the integration analysis of metabolomics, transcriptomics and biochemical approaches.
- Metabolomic analysis as collaborative projects with internal and external groups for a variety of plant functions with Arabidopsis thaliana and crops.
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