Novel methods for measurement of living systems are making new breakthroughs in life science. Nuclear Magnetic Resonance(NMR) can measure diverse biomolecules including soluble and insoluble states, if adequate nucleus are observed. Our laboratory intends to initiate metabolic analysis by developing new methods using an NMR-based approach, such as high quantification, non-invasive measurements, localized spectroscopy in vivo, selectivity of nuclear environments, versatility of pre- treatments of the samples, and validity of structure analysis of diverse biomolecules including stereo-isomers. This will be achieved by uniform stable isotope labeling of higher plants allowing application of multi-dimensional NMR experiments used in protein structure determination. Furthermore, we will develop the methods for metabolic dynamics and network analysis toward plant biomass utilization, for better improvements of food nutrition and global environment.

1. Advancement of the uniform stable isotope labeling methodology in living tissues

2.Technological advancement of solution / solid-state NMR measurements for studies of metabolite mixtures

3. Development of the methods for metabolic dynamics and network analysis toward plant biomass utilization